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Cloning, localization and differential expression of the Trypanosoma cruzi TcOGNT-2 glycosyl transferase
- Source :
- Gene, Gene, Elsevier, 2012, 498 (2), pp.147-54. ⟨10.1016/j.gene.2012.02.018⟩
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- International audience; The surface of Trypanosoma cruzi is covered by a dense glycocalix which is characteristic of each stage of the life cycle. Its composition and complexity depend mainly on mucin-like proteins. A remarkable feature of O-glycan biosynthesis in trypanosomes is that it initiates with the addition of a GlcNAc instead of the GalNAc residue that is commonly used in vertebrate mucins. The fact that the interplay between trans-sialidase and mucin is crucial for pathogenesis, and both families have stage-specific members is also remarkable. Recently the enzyme that transfers the first GlcNAc from UDP-GlcNAc to a serine or threonine residue was kinetically characterized. The relevance of this enzyme is evidenced by its role as catalyzer of the first step in O-glycosylation. In this paper we describe how this gene is expressed differentially along the life cycle with a pattern that is very similar to that of trans-sialidases. Its localization was determined, showing that the protein predicted to be in the Golgi apparatus is also present in reservosomes. Finally our results indicate that this enzyme, when overexpressed, enhances T. cruzi infectivity.
- Subjects :
- Chagas disease
Trypanosoma cruzi
Glycosyl-transferase
Golgi Apparatus
Neuraminidase
Biology
Gene Expression Regulation, Enzymologic
Uridine Diphosphate
Acetylglucosamine
Serine
03 medical and health sciences
symbols.namesake
Differential expression
Chlorocebus aethiops
Glycosyltransferase
Genetics
Animals
Cloning, Molecular
Threonine
3' Untranslated Regions
Vero Cells
Gene
Glycoproteins
030304 developmental biology
O-glycosylation
chemistry.chemical_classification
Life Cycle Stages
0303 health sciences
030302 biochemistry & molecular biology
Mucin
Glycosyltransferases
General Medicine
Golgi apparatus
biology.organism_classification
carbohydrates (lipids)
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Enzyme
Infectivity
chemistry
Biochemistry
biology.protein
symbols
Rabbits
Subjects
Details
- ISSN :
- 03781119 and 18790038
- Volume :
- 498
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....6e645f7ded2efc18993dc86759dfc100