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Identification of a super-functional Tfh-like subpopulation in murine lupus by pattern perception
- Source :
- eLife, Vol 9 (2020), eLife
- Publication Year :
- 2020
- Publisher :
- eLife Sciences Publications Ltd, 2020.
-
Abstract
- Dysregulated cytokine expression by T cells plays a pivotal role in the pathogenesis of autoimmune diseases. However, the identification of the corresponding pathogenic subpopulations is a challenge, since a distinction between physiological variation and a new quality in the expression of protein markers requires combinatorial evaluation. Here, we were able to identify a super-functional follicular helper T cell (Tfh)-like subpopulation in lupus-prone NZBxW mice with our binning approach "pattern recognition of immune cells (PRI)". PRI uncovered a subpopulation of IL-21+ IFN-γhigh PD-1low CD40Lhigh CXCR5- Bcl-6- T cells specifically expanded in diseased mice. In addition, these cells express high levels of TNF-α and IL-2, and provide B cell help for IgG production in an IL-21 and CD40L dependent manner. This super-functional T cell subset might be a superior driver of autoimmune processes due to a polyfunctional and high cytokine expression combined with Tfh-like properties.
- Subjects :
- 0301 basic medicine
Mouse
Autoimmunity
Lymphocyte Activation
CXCR5
Pattern Recognition, Automated
Pathogenesis
Mice
Immunology and Inflammation
0302 clinical medicine
T-Lymphocyte Subsets
Lupus Erythematosus, Systemic
Biology (General)
cd4+ t cells
B-Lymphocytes
Mice, Inbred NZB
biology
General Neuroscience
T-Lymphocytes, Helper-Inducer
General Medicine
Flow Cytometry
Cell biology
medicine.anatomical_structure
Medicine
Female
medicine.symptom
multi-parametric analysis
Research Article
combinatorial protein expression
QH301-705.5
T cell
Science
Inflammation
autoimmune disease
General Biochemistry, Genetics and Molecular Biology
high-dimensional flow cytometry
03 medical and health sciences
Immune system
medicine
Animals
cytokine expression
B cell
Autoimmune disease
CD40
General Immunology and Microbiology
Interleukins
Cell Biology
medicine.disease
Antigens, Differentiation
Coculture Techniques
030104 developmental biology
biology.protein
Immunologic Memory
030215 immunology
Subjects
Details
- Language :
- English
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- eLife
- Accession number :
- edsair.doi.dedup.....6e6696c967686ba26d7bc35c2bd3d838