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Continuous evolution of B. thuringiensis toxins overcomes insect resistance
- Source :
- Nature
- Publication Year :
- 2016
-
Abstract
- The Bacillus thuringiensis δ-endotoxins (Bt toxins) are widely used insecticidal proteins in engineered crops that provide agricultural, economic, and environmental benefits. The development of insect resistance to Bt toxins endangers their long-term effectiveness. Here we have developed a phage-assisted continuous evolution selection that rapidly evolves high-affinity protein-protein interactions, and applied this system to evolve variants of the Bt toxin Cry1Ac that bind a cadherin-like receptor from the insect pest Trichoplusia ni (TnCAD) that is not natively bound by wild-type Cry1Ac. The resulting evolved Cry1Ac variants bind TnCAD with high affinity (dissociation constant Kd = 11-41 nM), kill TnCAD-expressing insect cells that are not susceptible to wild-type Cry1Ac, and kill Cry1Ac-resistant T. ni insects up to 335-fold more potently than wild-type Cry1Ac. Our findings establish that the evolution of Bt toxins with novel insect cell receptor affinity can overcome insect Bt toxin resistance and confer lethality approaching that of the wild-type Bt toxin against non-resistant insects.
- Subjects :
- 0301 basic medicine
Insecticides
bacterial 2-hybrid
Plasma protein binding
Insect
Moths
medicine.disease_cause
Insecticide Resistance
Hemolysin Proteins
Bacillus thuringiensis
Trichoplusia
Bacteriophages
media_common
agriculture
Multidisciplinary
biology
Cell Death
Protein Stability
Cadherins
Plants, Genetically Modified
Genetically modified organism
Biotechnology
Protein Binding
Crops, Agricultural
media_common.quotation_subject
Molecular Sequence Data
Mutagenesis (molecular biology technique)
plant biotechnology
Article
Microbiology
03 medical and health sciences
Bacillus thuringiensis toxin
Bacterial Proteins
Consensus Sequence
medicine
Animals
Amino Acid Sequence
Selection, Genetic
protein evolution
Pest Control, Biological
030102 biochemistry & molecular biology
Bacillus thuringiensis Toxins
Toxin
business.industry
fungi
insecticide
Genetic Variation
PACE
biology.organism_classification
Endotoxins
030104 developmental biology
Cry1Ac
Mutagenesis
insect resistance
Directed Molecular Evolution
business
Subjects
Details
- Language :
- English
- ISSN :
- 14764687 and 00280836
- Volume :
- 533
- Issue :
- 7601
- Database :
- OpenAIRE
- Journal :
- Nature
- Accession number :
- edsair.doi.dedup.....6e713e9e589c6d7566ab51c4e0de71a9