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Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler Chicks
- Source :
- Journal of Integrative Agriculture, Vol 11, Iss 7, Pp 1173-1180 (2012)
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- Different amino acids have been shown to affect feed intake when injected directly into the central nervous system of birds. In the present study, we investigated the effects of L-glutamine and L-alanine on feed intake and the mRNA expression levels of hypothalamic neuropeptides involved in feed intake regulation in broiler chicks. L-Glutamine or L-alanine was intra-cerebroventricularly (ICV) administered to 4-d-old broiler chicks and the feed intake were recorded at various time points. Quantitative PCR was performed to determine the hypothalamic mRNA expression levels of neuropeptide Y (NPY), agouti related protein (AgRP), pro-opiomelanocortin (POMC), melanocortin receptor 4 (MC4R) and corticotropin releasing factor (CRF). Our results showed that ICV administration of L-glutamine (0.55 or 5.5 μmol) significantly increased feed intake up to 2 h post-administration period and the hypothalamic NPY mRNA expression levels, while it markedly decreased hypothalamic POMC and CRF mRNA expression levels. In contrast, ICV administration of L-alanine (4 μmol) significantly decreased feed intake for the first 0.5 h post-administration period, and reduced the hypothalamic AgRP mRNA expression levels, while it remarkablely enhanced the mRNA expression levels of MC4R and CRF. These findings suggested that L-glutamine and L-alanine could act within the hypothalamus to influence feed intake in broiler chicks, and that both orexigenic and anorexigenic neuropeptide genes might contribute directly to these effects.
- Subjects :
- medicine.medical_specialty
Agriculture (General)
Central nervous system
L-glutamine
Neuropeptide
Plant Science
Biology
Biochemistry
S1-972
intracerebroventricular (ICV)
Food Animals
Orexigenic
Internal medicine
medicine
hypothalamus
Ecology
digestive, oral, and skin physiology
Broiler
Neuropeptide Y receptor
Glutamine
Melanocortin 4 receptor
Endocrinology
medicine.anatomical_structure
Hypothalamus
L-alanine
feed intake
Animal Science and Zoology
Agronomy and Crop Science
hormones, hormone substitutes, and hormone antagonists
Food Science
medicine.drug
Subjects
Details
- ISSN :
- 20953119
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Journal of Integrative Agriculture
- Accession number :
- edsair.doi.dedup.....6e718a4670d7f09e4fb4e7190323d07d