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An Integrated Analysis of mRNA and lncRNA Expression Profiles Indicates Their Potential Contribution to Brown Fat Dysfunction With Aging
- Source :
- Frontiers in Endocrinology, Frontiers in Endocrinology, Vol 11 (2020)
- Publication Year :
- 2019
-
Abstract
- Brown adipose tissue (BAT) can convert fatty acids and glucose into heat, exhibiting the potential to combat obesity and diabetes. The mass and activity of BAT gradually diminishes with aging. As a newly found regulator of gene expression, long non-coding RNAs (lncRNAs) exhibit a wide range of functions in life processes. However, whether long non-coding RNA (lncRNA) involves in BAT dysfunction with aging is still unclear. Here, using RNA-sequencing technology, we identified 3237 messenger RNAs (mRNAs) and 1312 lncRNAs as differentially expressed in BAT of 10-months-old mice compared with 6- to 8-week-old. The protein-protein interaction network and k-score analysis revealed that the core mRNAs were associated with two important aging-related pathways, including cell cycle and p53 signaling pathway. Gene set enrichment analysis indicated that these mRNAs might participate in lipid metabolism and brown fat dysfunction. Functional enrichment analyses demonstrated that dysregulated lncRNAs were associated with mitochondria, regulation of cellular senescence, cell cycle, metabolic and p53 signaling pathways. Moreover, we revealed that two lncRNAs (NONMMUT024512 and n281160) may involve in the regulation of their adjacent gene peroxisome proliferator-activated receptor alpha (Pparα), a thermogenesis regulator. Collectively, these results lay a foundation for extensive studies on the role of lncRNAs in age-related thermogenic degradation.
- Subjects :
- 0301 basic medicine
Male
Aging
Endocrinology, Diabetes and Metabolism
Regulator
RNA-sequencing
030209 endocrinology & metabolism
Mitochondrion
Biology
lcsh:Diseases of the endocrine glands. Clinical endocrinology
03 medical and health sciences
Mice
0302 clinical medicine
Endocrinology
Adipose Tissue, Brown
Gene expression
Brown adipose tissue
medicine
Animals
Gene Regulatory Networks
Protein Interaction Maps
RNA, Messenger
BAT dysfunction
Gene
Pparα
Original Research
Messenger RNA
lcsh:RC648-665
Sequence Analysis, RNA
Gene Expression Profiling
RNA
thermogenesis
Cell biology
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
RNA, Long Noncoding
Transcriptome
Thermogenesis
GSEA analysis
Signal Transduction
Subjects
Details
- ISSN :
- 16642392
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Frontiers in endocrinology
- Accession number :
- edsair.doi.dedup.....6e76d5384814b17210e196edb5a0d35e