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Salvia miltiorrhiza protects against diabetic nephropathy through metabolome regulation and wnt/β-catenin and TGF-β signaling inhibition
- Source :
- Pharmacological Research. 139:26-40
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Diabetic nephropathy (DN) is a complication of diabetes that is caused by uncontrolled high blood sugar. It has been reported that Salvia miltiorrhiza (SM) possesses the ability to prevent kidney damage, although the mechanisms remain unclear. The study was to investigate whether and how SM improved DN injury via regulation of metabolome and the molecular mechanisms. In this study, SD rats were fed a high glucose / high fat diet accompanied by 0.5% glucose water. Three weeks later, the rats were given one intraperitoneal injection of 30 mg/kg STZ each day for three days for DN model. The biochemical indicators and metabolomics of plasma, urine and renal tissue were analyzed. Then the western blotting analysis of renal tissue and glomerular mesangial cells were investigated. The results showed that Salvia miltiorrhiza extracts improved the renal injury and regulation of abnormal glycolipid metabolism. The metabolites in serum, urine and renal tissues have been changed significantly. The involved metabolic pathways mainly include phospholipid, arachidonic acid, and pyrimidine metabolisms. Meanwhile, SM inhibited the relative expression levels of wnt4, β-catenin and TGF-β in renal tissue and high-glucose induced glomerular mesangial cells.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
medicine.medical_treatment
Glomerular Mesangial Cell
Intraperitoneal injection
Blood sugar
Salvia miltiorrhiza
Kidney
Protective Agents
Rats, Sprague-Dawley
Diabetic nephropathy
03 medical and health sciences
0302 clinical medicine
Transforming Growth Factor beta
Internal medicine
Diabetes mellitus
medicine
Metabolome
Animals
Diabetic Nephropathies
beta Catenin
Pharmacology
Plant Extracts
Chemistry
medicine.disease
Wnt Proteins
030104 developmental biology
medicine.anatomical_structure
Endocrinology
030220 oncology & carcinogenesis
Signal Transduction
Subjects
Details
- ISSN :
- 10436618
- Volume :
- 139
- Database :
- OpenAIRE
- Journal :
- Pharmacological Research
- Accession number :
- edsair.doi.dedup.....6e97eda9dcf513ed274e6680355fba67