Back to Search
Start Over
MRI-sensitive contrast agent with anticoagulant activity for surface camouflage of transplanted pancreatic islets
MRI-sensitive contrast agent with anticoagulant activity for surface camouflage of transplanted pancreatic islets
- Source :
- Biomaterials. 138
- Publication Year :
- 2017
-
Abstract
- Pancreatic islet implantation in the liver is a promising approach for diabetes therapy. However, 70% of the islet mass fails to be engrafted in the liver due to the instant blood-mediated inflammatory reactions (IBMIR) resulting from direct contact between islet cells and the bloodstream. To overcome this issue, direct monitoring is very important for establishing prognosis after islet cell therapy. Here we established a new type of MR contrast agent with anticoagulant activity via heparin-immobilized superparamagnetic iron oxide (HSPIO). The HSPIO was chemically conjugated onto islet surface ex vivo without damage of their viability and functionality. The conjugated HSPIO nanoparticles onto islet surface could attenuate IBMIR in vitro and in vivo. The HSPIO-conjugated islets could cure the blood glucose levels of diabetes animals after implantation. In addition, the HSPIO nanoparticles were well maintained on the transplanted islets for a long time during modulation of inflammation. Also, they allowed for stable visualization of the implanted islet cells for more than 150 days without reduction of the MRI signal. Furthermore, when HSPIO itself was intraportally injected, it was rapidly eliminated without accumulation in the liver, suggesting that HSPIO nanoparticles could only track the immobilized islet. Collectively, this HSPIO nanoparticle having MRI sensitivity and anticoagulant activity could be utilized for successful islet implantation.
- Subjects :
- 0301 basic medicine
Blood Glucose
Male
Time Factors
endocrine system diseases
Islets of Langerhans Transplantation
Contrast Media
030230 surgery
Ferric Compounds
Cell therapy
Rats, Sprague-Dawley
Mice
0302 clinical medicine
Magnetite Nanoparticles
geography.geographical_feature_category
Heparin
Islet
Prognosis
Magnetic Resonance Imaging
medicine.anatomical_structure
Liver
Mechanics of Materials
medicine.symptom
medicine.drug
endocrine system
medicine.medical_specialty
Materials science
Biophysics
Mice, Nude
Bioengineering
Inflammation
Diabetes Mellitus, Experimental
Biomaterials
03 medical and health sciences
Islets of Langerhans
In vivo
Internal medicine
medicine
Animals
Humans
Monitoring, Physiologic
geography
Pancreatic islets
Anticoagulants
Rats
Transplantation
030104 developmental biology
Endocrinology
Ceramics and Composites
Cancer research
Ex vivo
Subjects
Details
- ISSN :
- 18785905
- Volume :
- 138
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....6ef249bbbd93db7e36c89db0ac05de75