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The role of mesenteric lymph exosomal lipid mediators following intestinal ischemia-reperfusion injury on activation of inflammation
- Source :
- Journal of Trauma and Acute Care Surgery. 89:1099-1106
- Publication Year :
- 2020
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2020.
-
Abstract
- Background Intestinal ischemia caused by hemorrhagic shock is known to induce systemic inflammatory responses. Previous studies have shown that mesenteric lymph (ML) plays a crucial role in gut-mediated inflammation. Lipid mediators, such as lysophosphatidylcholines (LPCs) which contain polyunsaturated fatty acids (PUFAs), are present in the post-shock ML. Exosomes are also present in the ML and act as transcellular carriers of lipids; however, their role in post-shock systemic inflammation has not been revealed. Here, we aimed to identify changes in lipid mediators in ML exosomes after intestinal ischemia. Methods Male Sprague-Dawley rats underwent laparotomy, followed by ML duct cannulation. Animals were subjected to 60 min of intestinal ischemia by superior mesenteric artery clamping, followed by 120 min of reperfusion. ML was obtained before and after intestinal ischemia and exosomes were isolated from ML by ultracentrifugation. The biological activity of ML exosomes was determined using the monocyte NF-κB activation assay. Lipids of ML exosomes were extracted and quantified by liquid chromatography/electrospray ionization mass spectrometry. Results ML exosomes-induced NF-κB activation significantly increased after intestinal ischemia and lipid analysis revealed a significant increase in the concentration of PUFA-containing LPCs. Additionally, PUFA-containing LPCs also induced NF-κB activation. Conclusion Our results suggest that biologically active lipid mediators in ML exosomes may be involved in the inflammatory response after intestinal ischemia. Level of evidence Not applicable (Basic Science paper).
- Subjects :
- Male
Inflammation
Shock, Hemorrhagic
Pharmacology
Exosomes
Critical Care and Intensive Care Medicine
Systemic inflammation
Monocytes
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
medicine.artery
medicine
Animals
Mesentery
Superior mesenteric artery
Transcellular
Lymphatic Vessels
business.industry
Monocyte
NF-kappa B
030208 emergency & critical care medicine
Lipid signaling
medicine.disease
Rats
Disease Models, Animal
medicine.anatomical_structure
Reperfusion Injury
Surgery
Lymph
medicine.symptom
business
Reperfusion injury
Subjects
Details
- ISSN :
- 21630763 and 21630755
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- Journal of Trauma and Acute Care Surgery
- Accession number :
- edsair.doi.dedup.....6efd4650b2e419d50d7fcd8b1dd3be3d