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Pifithrin‐α Enhances the Survival of Transplanted Neural Stem Cells in Stroke Rats by Inhibiting p53 Nuclear Translocation

Authors :
Wenzhong Du
Yongli Li
Cong Liu
Xianfeng Li
Xu Sun
Shi-Yue Xin
Ying Sun
Xu-Hui Lei
Dan Zhao
Zhi-Ren Zhang
Chuanlu Jiang
Zhongfei Hao
Source :
CNS Neuroscience & Therapeutics. 19:109-116
Publication Year :
2012
Publisher :
Wiley, 2012.

Abstract

Summary Aims To examine a novel strategy to enhance the survival of grafted neural stem cells (NSCs) in stroke model. Methods Using a cell counting kit-8 (CCK-8) and TUNEL assay to test the protective effects of p53 inhibitor, pifithrin-α (PFT-α), on oxygen glucose deprivation (OGD) in NSCs. We compared the effects of vehicle + NSCs and FFT-α + NSCs on the efficacy of transplantation in stroke rat model using behavioral analysis, immunohistochemistry, etc. Results Pifithrin-α increased viability and decreased apoptosis in NSCs after OGD in vitro. By in vivo studies, we showed that the best recovery of neurological function in the stroke rats and the maximum survival of grafted NSCs were found in the PFT-α + NSCs group. Twelve hours after cell transplantation, p53 was localized to the nuclei of grafted NSCs in the vehicle + NSCs group but was primarily localized to the cytoplasm in the PFT-α + NSCs group. The p53-upregulated modulator of apoptosis (PUMA) was highly expressed among the grafted cells in the vehicle + NSCs group compared with that in the PFT-α + NSCs group. Conclusion Our results indicate that PFT-α enhances the survival of grafted NSCs through the inhibition of p53 translocation into the nucleus.

Details

ISSN :
17555949 and 17555930
Volume :
19
Database :
OpenAIRE
Journal :
CNS Neuroscience & Therapeutics
Accession number :
edsair.doi.dedup.....6f146a604905609b7450caa96d5be988