Back to Search Start Over

Targeting SKA3 suppresses the proliferation and chemoresistance of laryngeal squamous cell carcinoma via impairing PLK1–AKT axis-mediated glycolysis

Authors :
Yongyan Wu
Yunfeng Bo
Guo Huina
Yan Lu
Yujia Guo
Rui Hou
Min Niu
Changming An
Jiajia Cui
Xu Wei
Wei Gao
Lei Jin
Tao Yang
Shuxin Wen
Yu Zhang
Teng Ma
Li Li
Dai Fengsheng
Linshi Zhang
Hongjie Luo
Yuliang Zhang
Hu Wanglai
Zheng Xiwang
Dongli Yang
Huizheng Li
Xue Xuting
Source :
Cell Death and Disease, Vol 11, Iss 10, Pp 1-19 (2020), Cell Death & Disease
Publication Year :
2020
Publisher :
Springer Science and Business Media LLC, 2020.

Abstract

Spindle and kinetochore-associated complex subunit 3 (SKA3) is a well-known regulator of chromosome separation and cell division, which plays an important role in cell proliferation. However, the mechanism of SKA3 regulating tumor proliferation via reprogramming metabolism is unknown. Here, SKA3 is identified as an oncogene in laryngeal squamous cell carcinoma (LSCC), and high levels of SKA3 are closely associated with malignant progression and poor prognosis. In vitro and in vivo experiments demonstrate that SKA3 promotes LSCC cell proliferation and chemoresistance through a novel role of reprogramming glycolytic metabolism. Further studies reveal the downstream mechanisms of SKA3, which can bind and stabilize polo-like kinase 1 (PLK1) protein via suppressing ubiquitin-mediated degradation. The accumulation of PLK1 activates AKT and thus upregulates glycolytic enzymes HK2, PFKFB3, and PDK1, resulting in enhancement of glycolysis. Furthermore, our data reveal that phosphorylation at Thr360 of SKA3 is critical for its binding to PLK1 and the increase in glycolysis. Collectively, the novel oncogenic signal axis “SKA3-PLK1-AKT” plays a critical role in the glycolysis of LSCC. SKA3 may serve as a prognostic biomarker and therapeutic target, providing a potential strategy for proliferation inhibition and chemosensitization in tumors, especially for LSCC patients with PLK1 inhibitor resistance.

Details

ISSN :
20414889
Volume :
11
Database :
OpenAIRE
Journal :
Cell Death & Disease
Accession number :
edsair.doi.dedup.....6f505bdc7f6f06518df50ac03a75a64b