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Differential lactate and cholesterol synthetic activities in XY and XX Sertoli cells
- Source :
- Scientific Reports
- Publication Year :
- 2017
- Publisher :
- Nature Research, 2017.
-
Abstract
- SRY, a sex-determining gene, induces testis development in chromosomally female (XX) individuals. However, mouse XX Sertoli cells carrying Sry (XX/Sry Sertoli cells) are incapable of fully supporting germ cell development, even when the karyotype of the germ cells is XY. While it has therefore been assumed that XX/Sry Sertoli cells are not functionally equivalent to XY Sertoli cells, it has remained unclear which specific functions are affected. To elucidate the functional difference, we compared the gene expression of XY and XX/Sry Sertoli cells. Lactate and cholesterol metabolisms, essential for nursing the developing germ cells, were down-regulated in XX/Sry cells, which appears to be caused at least in part by the differential expression of histone modification enzymes SMCX/SMCY (H3K4me3 demethylase) and UTX/UTY (H3K27me3 demethylase) encoded by the sex chromosomes. We suggest that down-regulation of lactate and cholesterol metabolism that may be due to altered epigenetic modification affects the nursing functions of XX/Sry Sertoli cells.<br />This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number 21249018 and 16H05142 (K. Mo.), Ministry of Education, Culture, Sports, Science, and Technology, Japan (MEXT) KAKENHI Grant Number 22132002 (K. Mo.), the Uehara Memorial Foundation, and Takeda Science Foundation (T.B.).
- Subjects :
- 0301 basic medicine
Male
endocrine system
X Chromosome
Karyotype
Disorders of Sex Development
Article
03 medical and health sciences
Mice
Y Chromosome
medicine
Animals
Epigenetics
Lactic Acid
Cells, Cultured
Histone Demethylases
Multidisciplinary
Sertoli Cells
biology
social sciences
Sertoli cell
Sex-Determining Region Y Protein
Cell biology
Histone Code
030104 developmental biology
Histone
medicine.anatomical_structure
Testis determining factor
Cholesterol
biology.protein
H3K4me3
Demethylase
Female
Germ cell
geographic locations
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....6f812a43c5fcf51c40a21b16bf86afae