Ecotoxicological risk assessment of the 'Acid Black 210' dye

Made available in DSpace on 2018-11-26T17:55:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-02-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The Acid Black 210 dye is one of the most used black dyes by the leather industry. This compound contains three azo groups in its chemical structure, and has been quoted as a non-regulated dye with toxicological concern, since it could generate carcinogenic aromatic amines. The objective of this study was to perform the ecotoxicological risk assessment of this dye through testing its toxicity in vitro and in vivo with the Ames test, the Comet assay, the Daphnia similis test, and the zebrafish embryo acute toxicity test. Moreover, we evaluated the presence of this dye in environmental samples related with a tannery industry. All the tests performed were negative, with the exception of the Ames test with the Salmonella typhimurium TA98 strain, which resulted in a low mutagenic potency. Due to the low concentrations of the Acid Black 210 dye found in tannery effluents, and the high concentrations where any toxic activity is occasionally described, we concluded that this dye is safe from the ecotoxicological point of view in the areas evaluated and in the light of the current knowledge. (C) 2016 Elsevier Ireland Ltd. All rights reserved. Univ Sao Paulo, Lab Environm Toxicol, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP, Brazil Univ Estadual Paulista, Inst Chem, BR-14800060 Araraquara, SP, Brazil Univ Sao Paulo, Sch Pharmaceut Sci, Skin Biol Lab, BR-05508000 Sao Paulo, SP, Brazil Univ Estadual Paulista, Inst Chem, BR-14800060 Araraquara, SP, Brazil FAPESP: 2008/10449-7 FAPESP: 2013/09317-7 FAPESP: 2013/06172-8 FAPESP: 2013/24340-5