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Life under the Microscope: Single-Molecule Fluorescence Highlights the RNA World
- Source :
- Chemical reviews. 118(8)
- Publication Year :
- 2018
-
Abstract
- The emergence of single-molecule (SM) fluorescence techniques has opened up a vast new toolbox for exploring the molecular basis of life. The ability to monitor individual biomolecules in real time enables complex, dynamic folding pathways to be interrogated without the averaging effect of ensemble measurements. In parallel, modern biology has been revolutionized by our emerging understanding of the many functions of RNA. In this comprehensive review, we survey SM fluorescence approaches and discuss how the application of these tools to RNA and RNA-containing macromolecular complexes in vitro has yielded significant insights into the underlying biology. Topics covered include the three-dimensional folding landscapes of a plethora of isolated RNA molecules, their assembly and interactions in RNA-protein complexes, and the relation of these properties to their biological functions. In all of these examples, the use of SM fluorescence methods has revealed critical information beyond the reach of ensemble averages.
- Subjects :
- 0301 basic medicine
Riboswitch
Transcription, Genetic
RNA Splicing
Computational biology
Catalysis
Fluorescence
Article
03 medical and health sciences
Transcription (biology)
Fluorescence Resonance Energy Transfer
Telomerase
chemistry.chemical_classification
Gene Editing
Biomolecule
RNA
RNA-Binding Proteins
General Chemistry
Reverse Transcription
Single-molecule experiment
Folding (chemistry)
030104 developmental biology
Förster resonance energy transfer
Spectrometry, Fluorescence
chemistry
Microscopy, Fluorescence
Protein Biosynthesis
RNA splicing
Nucleic Acid Conformation
RNA Interference
CRISPR-Cas Systems
Subjects
Details
- ISSN :
- 15206890
- Volume :
- 118
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Chemical reviews
- Accession number :
- edsair.doi.dedup.....6fec63f81fe1c4fb9c2b9499265b1219