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A proteomic analysis of serum-derived exosomes in rheumatoid arthritis
- Source :
- BMC Rheumatology, Vol 2, Iss 1, Pp 1-11 (2018), BMC rheumatology
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Background To understand the roles of serum exosomes in rheumatoid arthritis (RA), we comprehensively investigated the protein profiles of serum exosomes in patients with RA. Methods Exosomes were isolated from serum samples obtained from 33 patients (12 with active RA [aRA], 11 with inactive RA [iRA], 10 with osteoarthritis [OA]) and 10 healthy donors (HLs). Proteins extracted from the exosomes were separated by two-dimensional differential gel electrophoresis (2D-DIGE) and identified by mass spectrometry. Results In total, 204 protein spots were detected by 2D-DIGE. In the aRA, iRA, and OA groups, 24, 5, and 7 spots showed approximately ≥ ±1.3-fold intensity differences compared with the HL group, respectively. We were able to identify proteins in six protein spots. Among them, the protein spot identified as Toll-like receptor 3 (TLR3) showed approximately 6-fold higher intensity in the aRA group than in the other groups. Conclusions Patients with active RA possessed considerably different protein profiles of serum exosomes from patients with iRA, patients with OA, and healthy donors. The unique protein profile of serum exosomes, such as the possession of abundant TLR3 fragments, may reflect the pathophysiology of active RA. Electronic supplementary material The online version of this article (10.1186/s41927-018-0041-8) contains supplementary material, which is available to authorized users.
- Subjects :
- Proteomics
0301 basic medicine
medicine.medical_specialty
lcsh:Diseases of the musculoskeletal system
Exosome
03 medical and health sciences
0302 clinical medicine
Rheumatology
Internal medicine
Osteoarthritis
medicine
Rheumatoid arthritis
Receptor
030203 arthritis & rheumatology
Gel electrophoresis
Chemistry
medicine.disease
Molecular biology
Pathophysiology
Microvesicles
Toll like receptor 3
030104 developmental biology
lcsh:RC925-935
Research Article
Subjects
Details
- ISSN :
- 25201026
- Volume :
- 2
- Database :
- OpenAIRE
- Journal :
- BMC Rheumatology
- Accession number :
- edsair.doi.dedup.....7016dc7e1d6b71e0fc4f7d6c4370bc9d