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Modification of the gravitropic response of seedling roots of rapeseed (Brassica napus) transformed by Agrobacterium rhizogenes A4
- Source :
- Physiologia Plantarum, Physiologia Plantarum, 1994, 91 (4), pp.559-566, Physiologia Plantarum, Wiley, 1994, 91, pp.559-566, Physiologia Plantarum, Wiley, 1994, 91 (4), pp.559-566
- Publication Year :
- 1994
- Publisher :
- Wiley, 1994.
-
Abstract
- International audience; Modification of the gravitropic response of seedling roots of rapeseed {Brassica napus) transformed by Agrobacterium rhizogenes A4 V. Legue, F. Vilaine, M, Tepfer and G, Perbal Legue. V,. Vilaine, E. Tepler. M. and Perbal. G. 1994. Modification ofthe gravitropic response of seedling roots of rapeseed \Bra.ssica napii.\i transformed by Agrobacieiium rhi:ngem's A4,-Physiol, Plant, 91: 559-566. We have e.xamlued the growth and gra\ itropic response of seedling roots of rapeseed {Brassica napus. CrGC5-l) transformed by AgrobacWiiiini rhizogenes .\4. in order to evaluate if this could constitute a new model system for the study of gravitropism. The transformed clone chosen for study had integrated full-length TL-and TR-D.NA from pRi (the root inducing piasmid). and thus included all of the agrobacteriai genes potentially involved in the modified phenotype of transformed plants. In the vertical position, the gnnvth rate of transformed roots was higher than controls. During 24 h of continuous stimulation, the optimal angle for gravitropic bending in normal roots was 135° twith respect to the gravity a.sis). with decreasing response at 90° and 45^, Eor transformed roots, slight curvature developed at 45"' and at 90°. and stronger curvature was observed at 135. though transformed root tips never reached the vertical position. The minimum stimulation time necessary to elicit a response (presentation time) was also determined: it was significantly shorter in normal roots t80 si than in transformed ones * 120 s). The results show that pRi transformed roots are less sensitive to gravity than normal roots.
- Subjects :
- 0106 biological sciences
Rapeseed
Rhizobiaceae
Agrobacterium
Physiology
Gravitropism
Brassica
Continuous stimulation
Model system
Plant Science
01 natural sciences
[SDV.BV.BOT] Life Sciences [q-bio]/Vegetal Biology/Botanics
03 medical and health sciences
Botany
Genetics
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
[SDV.BV] Life Sciences [q-bio]/Vegetal Biology
COLZA
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
0303 health sciences
biology
Cell Biology
General Medicine
[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics
biology.organism_classification
Seedling
ADN T
010606 plant biology & botany
Subjects
Details
- ISSN :
- 13993054 and 00319317
- Volume :
- 91
- Database :
- OpenAIRE
- Journal :
- Physiologia Plantarum
- Accession number :
- edsair.doi.dedup.....704ba846043d7faebeed1b633fa9d35a