Additional file 7: of Modulation of Wnt/BMP pathways during corneal differentiation of hPSC maintains ABCG2-positive LSC population that demonstrates increased regenerative potential

Figure S4. (A) Additional characterization of OCT3/4, PAX6, CK14, and CK15 expression at d24 in the novel CnT-07+ENRC maintenance condition. (B) Characterization of Ki67/ABCG2 protein expression at d11, as well as after continued culture in CnT-07+ENRC or CnT-30 at d21. In both panels, cell nuclei counterstained with DAPI (blue) and scale bars, 100â Îźm. Data are presented for the hESC lines Regea11/013 (A) and Regea08/017 (B). (DOCX 5938 kb)