Back to Search
Start Over
Systematic in vitro biocompatibility studies of multimodal cellulose nanocrystal and lignin nanoparticles
- Source :
- Journal of Biomedical Materials Research Part A. 108:770-783
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Natural biopolymer nanoparticles (NPs), including nanocrystalline cellulose (CNC) and lignin, have shown potential as scaffolds for targeted drug delivery systems due to their wide availability, cost‐efficient preparation, and anticipated biocompatibility. Since both CNC and lignin can potentially cause complications in cell viability assays due to their ability to scatter the emitted light and absorb the assay reagents, we investigated the response of bioluminescent (CellTiter‐Glo®), colorimetric (MTT® and AlamarBlue®) and fluorometric (LIVE/DEAD®) assays for the determination of the biocompatibility of the multimodal CNC and lignin constructs in murine RAW 264.7 macrophages and 4T1 breast adenocarcinoma cell lines. Here, we have developed multimodal CNC and lignin NPs harboring the radiometal chelator DOTA (1,4,7,10‐tetraazacyclododecane‐1,4,7,10‐tetraacetic acid) and the fluorescent dye Cyanine 5 for the investigation of nanomaterial biodistribution in vivo with nuclear and optical imaging, which were then used as the model CNC and lignin nanosystems in the cell viability assay comparison. CellTiter‐Glo® based on the detection of ATP‐dependent luminescence in viable cells revealed to be the best assay for both nanoconstructs for its robust linear response to increasing NP concentration and lack of interference from either of the NP types. Both multimodal CNC and lignin NPs displayed low cytotoxicity and favorable interactions with the cell lines, suggesting that they are good candidates for nanosystem development for targeted drug delivery in breast cancer and for theranostic applications. Our results provide useful guidance for cell viability assay compatibility for CNC and lignin NPs and facilitate the future translation of the materials for in vivo applications.
- Subjects :
- 116 Chemical sciences
Biocompatible Materials
multimodal imaging probe
02 engineering and technology
Lignin
TOXICITY
Mice
chemistry.chemical_compound
BIODISTRIBUTION
PARTICLE-SHAPE
drug delivery system
Tissue Distribution
DRUG-DELIVERY
nanomaterials
AGENT
Metals and Alloys
021001 nanoscience & nanotechnology
CANCER
nanomedicine
317 Pharmacy
Drug delivery
FUNCTIONALIZATION
cytotoxicity
Nanomedicine
0210 nano-technology
Biodistribution
Materials science
Biocompatibility
Cell Survival
0206 medical engineering
Biomedical Engineering
Biomaterials
biocompatibility
lignin nanoparticle
Cell Line, Tumor
Animals
Humans
Viability assay
Cellulose
cellulose nanocrystal
cell viability
technology, industry, and agriculture
TRENDS
020601 biomedical engineering
SURFACE-CHARGE
nanocrystalline cellulose
RAW 264.7 Cells
chemistry
Targeted drug delivery
Ceramics and Composites
Biophysics
Nanoparticles
RESISTANCE
radiopharmaceutical chemistry
Subjects
Details
- ISSN :
- 15524965 and 15493296
- Volume :
- 108
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Materials Research Part A
- Accession number :
- edsair.doi.dedup.....70a930fd2e3c4ae4d055ad2248d4ec4b