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An AFLP marker linked to the leaf rust resistance gene LrBi16 and test of allelism with Lr14a on chromosome arm 7BL
- Source :
- Crop Journal, Vol 3, Iss 2, Pp 152-156 (2015)
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Leaf rust (LR), caused by Puccinia triticina , is one of the most widespread diseases of common wheat ( Triticum aestivum L.) worldwide. The LR resistance gene LrBi16 has been mapped on chromosome arm 7BL in Chinese wheat cultivar Bimai 16 and was closely linked to SSR loci Xcfa2257 and Xgwm344 with genetic distances of 2.8 cM and 2.9 cM, respectively. In the present study, a total of 304 AFLP primer pairs were used to screen Bimai 16 and Thatcher and resistant and susceptible DNA bulks. The polymorphic AFLP marker P-ATT/M-CGC 173 bp was used to genotype F 2 and F 3 populations to identify markers more closely linked to LrBi16 . Marker P-ATT/M-CGC 173 bp was tightly linked to LrBi16 with a genetic distance of 0.5 cM. As LrBi16 was mapped near the Lr14a locus, 809 F 2 plants from the Bimai 16/RL6013 ( Lr14a ) cross were inoculated with the Pt pathotype FHNQ to test the allelism of Lr14a and LrBi16 . All of the F 2 plants were resistant to FHNQ (IT between; and 2), suggesting that Lr14a and LrBi16 are allelic.
- Subjects :
- Genetics
lcsh:S
food and beverages
Puccinia triticina
Locus (genetics)
Plant Science
Biology
Genetic linkage
lcsh:S1-972
lcsh:Agriculture
Amplified fragment length polymorphism
Genetic distance
Chromosome Arm
Genotype
Molecular mapping
lcsh:Agriculture (General)
Common wheat
Allele
Agronomy and Crop Science
Subjects
Details
- ISSN :
- 22145141
- Volume :
- 3
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- The Crop Journal
- Accession number :
- edsair.doi.dedup.....70cb8fb8b0787be72a8301d13fbfc691
- Full Text :
- https://doi.org/10.1016/j.cj.2014.11.004