Back to Search
Start Over
The Gene Expression Profile of Cyst Epithelial Cells in Autosomal Dominant Polycystic Kidney Disease Patients
- Source :
- BMB Reports. 37:612-617
- Publication Year :
- 2004
- Publisher :
- Korean Society for Biochemistry and Molecular Biology - BMB Reports, 2004.
-
Abstract
- Autosomal dominant polycystic kidney disease (ADPKD) is a common genetic disorder characterized by the formation of fluid-filled cysts in the kidney and progressive renal failure. Other manifestations of ADPKD include the formation of cysts in other organs (liver, pancreas, and spleen), hypertension, cardiac defects, and cerebral aneurysms. The loss of function of the polycystin -1 and -2 results in the formation of epithelium-lined cysts, a process that depends on initial epithelial proliferation. cDNA microarrays powerfully monitor gene expression and have led to the discoveries of pathways regulating complex biological processes. We undertook to profile the gene expression patterns of epithelial cells derived from the cysts of ADPKD patients using the cDNA microarray technique. Candidate genes that were differently expressed in cyst tissues were identified. 19 genes were up-regulated, and 6 down-regulated. Semi-quantitative RT-PCR results were consistent with the microarray findings. To distinguish between normal and epithelial cells, we used the hierarchical method. The results obtained may provide a molecular basis for understanding the biological meaning of cytogenesis.
- Subjects :
- medicine.medical_specialty
Pathology
Candidate gene
TRPP Cation Channels
Microarray
Autosomal dominant polycystic kidney disease
Down-Regulation
Gene Expression
Biology
Biochemistry
Internal medicine
Gene expression
medicine
Humans
Cyst
Molecular Biology
Cells, Cultured
Kidney
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Genetic disorder
Membrane Proteins
Epithelial Cells
General Medicine
Polycystic Kidney, Autosomal Dominant
medicine.disease
Up-Regulation
medicine.anatomical_structure
Endocrinology
Pancreas
Subjects
Details
- ISSN :
- 19766696
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- BMB Reports
- Accession number :
- edsair.doi.dedup.....71138557558cb95138b5d79b94c695b0