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Gene and isoform expression signatures associated with tumor stage in kidney renal clear cell carcinoma
- Source :
- BMC Systems Biology
- Publication Year :
- 2014
-
Abstract
- Background Identification of expression alternations between early and late stage cancers is helpful for understanding cancer development and progression. Much research has been done focusing on stage-dependent gene expression profiles. In contrast, relatively fewer studies on isoform expression profiles have been performed due to the difficulty of quantification and noisy splicing. Here we conducted both gene- and isoform-level analysis on RNA-seq data of 234 stage I and 81 stage IV kidney renal clear cell carcinoma patients, aiming to uncover the stage-dependent expression signatures and investigate the advantage of isoform expression profiling for identifying advanced stage cancers and predicting clinical outcome. Results Both gene and isoform expression signatures are useful for distinguishing cancer stages. They provide common and unique information associated with cancer progression and metastasis. Combining gene and isoform signatures even improves the classification performance and reveals additional important biological processes, such as angiogenesis and TGF−beta signaling pathway. Moreover, expression abundance of a number of genes and isoforms is predictive of the risk of cancer death in an independent dataset, such as gene and isoform expression of ITPKA, the expression of a functional important isoform of UPS19. Conclusion Isoform expression profiling provides unique and important information which cannot be detected by gene expression profiles. Combining gene and isoform expression signatures helps to identify advanced stage cancers, predict clinical outcome, and present a comprehensive view of cancer development and progression.
- Subjects :
- Gene isoform
Male
Angiogenesis
Biology
Bioinformatics
Metastasis
03 medical and health sciences
0302 clinical medicine
Structural Biology
Modelling and Simulation
Gene expression
medicine
Humans
Protein Isoforms
Molecular Biology
Gene
Carcinoma, Renal Cell
030304 developmental biology
Neoplasm Staging
0303 health sciences
Applied Mathematics
Gene Expression Profiling
Research
Cancer
Middle Aged
medicine.disease
Prognosis
Kidney Neoplasms
3. Good health
Computer Science Applications
Gene expression profiling
030220 oncology & carcinogenesis
Modeling and Simulation
Cancer research
Female
Signal transduction
Genes, Neoplasm
Subjects
Details
- ISSN :
- 17520509
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- BMC systems biology
- Accession number :
- edsair.doi.dedup.....711f706e55ad17e64b1a5c53be8ed20d