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Gene delivery to tumor cells by cationic polymeric nanovectors coupled to folic acid and the cell-penetrating peptide octaarginine
- Source :
- Biomaterials. 32(29)
- Publication Year :
- 2011
-
Abstract
- Target ligand folic acid (FA) and cell-penetrating peptide octaarginine (R8) were coupled with the gene vectors (PEI 600 -CyD, PC) composed of β-cyclodextrin (β-CyD) and low-molecular-weight polyethylenimine (PEI, Mw 600) to form nanovectors for highly efficient gene delivery to tumor cells. The resultant ternary nanocomplexes of FA-PC/R8-PC/pDNA produced excellent gene transfaction abilities in the folate receptor (FR)-positive tumor cells in vitro and in vivo . The FR-mediated endocytosis and the R8-mediated transmembrane functionality together contributed to the high transfection levels. This study provides a promising means to produce gene nanovectors for in vivo applications.
- Subjects :
- Materials science
Polymers
Genetic Vectors
Biophysics
Mice, Nude
Bioengineering
Peptide
Biocompatible Materials
Cell-Penetrating Peptides
Gene delivery
Endocytosis
Transfection
Cell Line
Biomaterials
chemistry.chemical_compound
Mice
Folic Acid
Cations
Materials Testing
Animals
Humans
chemistry.chemical_classification
Polyethylenimine
Mice, Inbred BALB C
Molecular Structure
Gene Transfer Techniques
Genetic Therapy
Ligand (biochemistry)
Cell biology
Nanostructures
chemistry
Biochemistry
Mechanics of Materials
Folate receptor
Ceramics and Composites
Cell-penetrating peptide
Oligopeptides
Subjects
Details
- ISSN :
- 18785905
- Volume :
- 32
- Issue :
- 29
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....7125c13ddaf3b015b35a0b75456ba843