Back to Search
Start Over
Bacteroides thetaiotaomicron-infecting bacteriophage isolates inform sequence-based host range predictions
- Publication Year :
- 2020
- Publisher :
- Cold Spring Harbor Laboratory, 2020.
-
Abstract
- SummaryOur emerging view of the gut microbiome largely focuses on bacteria and less is known about other microbial components such as of bacteriophages (phages). Though phages are abundant in the gut, very few phages have been isolated from this ecosystem. Here, we report the genomes of 27 phages from the United States and Bangladesh that infect the prevalent human gut bacterium Bacteroides thetaiotaomicron. These phages are mostly distinct from previously sequenced phages with the exception of two, which are crAss-like phages. We compare these isolates to existing human gut metagenomes, revealing similarities to previously inferred phages and additional unexplored phage diversity. Finally, we use host tropisms of these phages to identify alleles of phage structural genes associated with infectivity. This work provides a detailed view of the gut’s “viral dark matter” and a framework for future efforts to further integrate isolation- and sequencing-focused efforts to understand gut-resident phages.
- Subjects :
- Comparative genomics
Genetics
Infectivity
0303 health sciences
Host (biology)
viruses
Structural gene
Biology
biology.organism_classification
Isolation (microbiology)
Microbiology
digestive system
Genome
Bacteriophage
03 medical and health sciences
0302 clinical medicine
Metagenomics
Virology
Parasitology
Bacteroides
Bacteroides thetaiotaomicron
030217 neurology & neurosurgery
Bacteria
030304 developmental biology
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....71584987e0f8c8ada808c491510b751c