Infection protocols, culturing conditions, measured fluoxetine concentrations and post-hoc analysis of fluoxetine reaction norms. from Can pharmaceutical pollution alter the spread of infectious disease? A case study using fluoxetine

Details on how the Daphnia were cultured and infected with Pasteuria ramosa spores during our experiment. The measured concentration of fluoxetine in water samples taken fortnightly from each fluoxetine treatment, performed using gas chromatography-tandem mass spectrometry. Post-hoc comparisons of the change in transmission and basic reproductive number between control and low fluoxetine treatment, and control and high fluoxetine treatments.