Reinnervation of motor units in intrinsic muscles of a transplanted hand

Functional recovery of transplanted hand can be evaluated clinically but until now there has been no direct assessment of muscle control. In October 2000 we transplanted the right hand of a brain-dead man aged 43 onto a man aged 35 who had lost his right dominant hand 22 years before. Starting from day 205 after the transplant, multi-channel surface electromyographic (EMG) signals were recorded from intrinsic muscles of the transplanted hand in order to assess their degree of reinnervation. Eleven months post-operatively, the first motor unit action potential train was detected from the abductor digiti minimi. One month later, also the abductor pollicis brevis and the opponens pollicis muscles showed motor unit activity, while, after 15 and 24 months, the first dorsal interosseous and the first lumbricalis muscles, respectively, showed activation of their first motor units. An increase in the number of active motor units was observed after the first signs of reinnervation, although the process was rather slow. In sustained maximal contractions, the motor unit discharge rate decreased from (mean +/- S.D.) 34.0+/-6.7 pps to 23.4+/-5.1 pps in 60 s for the abductor digiti minimi, although the subject was verbally encouraged to maintain a maximal activation. Moreover, the subject was able to perform basic control tasks involving voluntary modulation of motor unit discharge rate. With a visual feedback, he could increase discharge rate of the abductor digiti minimi approximately linearly over time, from 13.4+/-6.7 pps to 32.5+/-11.2 pps in 60 s. In conclusion, we showed reinnervation of single motor units in a transplanted hand after 22 years of denervation. Moreover, voluntary modulation of discharge rates of these motor units could be performed since the first sign of reinnervation.