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The GABABreceptor associates with regulators of G-protein signaling 4 protein in the mouse prefrontal cortex and hypothalamus
- Source :
- BMB Reports
- Publication Year :
- 2014
- Publisher :
- Korean Society for Biochemistry and Molecular Biology - BMB Reports, 2014.
-
Abstract
- Regulators of G-protein signaling (RGS) proteins regulate certain G-protein-coupled receptor (GPCR)-mediated signaling pathways. The GABAB receptor (GABABR) is a GPCR that plays a role in the stress response. Previous studies indicate that acute immobilization stress (AIS) decreases RGS4 in the prefrontal cortex (PFC) and hypothalamus (HY) and suggest the possibility of a signal complex composed of RGS4 and GABABR. Therefore, in the present study, we tested whether RGS4 associates with GABABR in these brain regions. We found the co-localization of RGS4 and GABABR subtypes in the PFC and HY using double immunohistochemistry and confirmed a direct association between GABAB2R and RGS4 proteins using co-immunoprecipitation. Furthermore, we found that AIS decreased the amount of RGS4 bound to GABAB2R and the number of double-positive cells. These results indicate that GABABR forms a signal complex with RGS4 and suggests that RGS4 is a regulator of GABABR. [BMB Reports 2014; 47(6): 324-329]
- Subjects :
- Male
medicine.medical_specialty
G protein
Immunoprecipitation
Hypothalamus
RGS4
Prefrontal Cortex
GABAB receptor
Biochemistry
Mice
Stress, Physiological
Internal medicine
medicine
Animals
Prefrontal cortex
Receptor
Molecular Biology
Research Articles
G protein-coupled receptor
biology
Stress response
General Medicine
Immunohistochemistry
Cell biology
Mice, Inbred C57BL
Endocrinology
Receptors, GABA-B
biology.protein
Signal transduction
RGS Proteins
Subjects
Details
- ISSN :
- 19766696
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- BMB Reports
- Accession number :
- edsair.doi.dedup.....71b630800b2dbe876b23a53f395e3abc