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Direct conversion of cheese whey to polymalic acid by mixed culture of Aureobasidium pullulans and permeabilized Kluyveromyces marxianus
- Source :
- Bioresource technology. 337
- Publication Year :
- 2021
-
Abstract
- Cheese whey is an abundant and low-cost feedstock with lactose as its main component, but the inability to metabolize lactose prevents Aureobasidium pullulans from using cheese whey directly. In this study, Kluyveromyces marxianus was permeabilized to obtain nonviable but biocatalytic cells for lactose hydrolysis, and the mixed culture of A. pullulans and permeabilized K. marxianus was conducted for polymalic acid (PMA) production from cheese whey. In the mixed culture, PMA titer varied directly to β-galactosidase activity of K. marxianus, but inversely to cell viability of K. marxianus, and ethanol permeabilized K. marxianus was the most compatible with A. pullulans for PMA production. 37.8 g/L PMA was produced in batch fermentation, and PMA titer was increased to 97.3 g/L in fed-batch fermentation, with a productivity of 0.51 g/(L·h) and a yield of 0.56 g/g. This study paved an economical and environmentally friendly way for PMA production from cheese whey.
- Subjects :
- 0106 biological sciences
Environmental Engineering
Polymers
Aureobasidium
Malates
Bioengineering
Lactose
010501 environmental sciences
Raw material
01 natural sciences
chemistry.chemical_compound
Kluyveromyces
Mixed culture
Kluyveromyces marxianus
Cheese
010608 biotechnology
Whey
Food science
Waste Management and Disposal
Glucans
0105 earth and related environmental sciences
Ethanol
biology
Renewable Energy, Sustainability and the Environment
food and beverages
General Medicine
biology.organism_classification
Polymalic acid
Aureobasidium pullulans
chemistry
Fermentation
Subjects
Details
- ISSN :
- 18732976
- Volume :
- 337
- Database :
- OpenAIRE
- Journal :
- Bioresource technology
- Accession number :
- edsair.doi.dedup.....71b94ba67dc9370cefa01cf7e2a9a3ed