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Mechanisms through which PDGF alters intracellular calcium levels in U-1242 MG human glioma cells
- Source :
- Neurochemistry International. 35:411-422
- Publication Year :
- 1999
- Publisher :
- Elsevier BV, 1999.
-
Abstract
- PDGF-BB induces a rapid, sustained increase in intracellular calcium levels in U-1242 MG cells. We used several calcium channel blockers to identify the types of channels involved. L channel blockers (verapamil, nimodipine, nicardipine, nitrendipine and taicatoxin) had no effect on PDGF-BB induced alterations in intracellular calcium. Blockers of P, Q and N channels (ω-agatoxin-IVA, ω-conotoxin MVIIC and ω-conotoxin GVIA) also had no effect. This indicates that these channels play an insignificant role in supplying the Ca 2+ necessary for PDGF stimulated events in U-1242 MG cells. However, a T channel blocker (NDGA) and the non-specific (NS) calcium channel blockers (FFA and SK&F 9365) abolished PDGF-induced increases in intracellular calcium. This indicates that PDGF causes calcium influx through both non-specific cationic channels and T channels. To study the participation of intracellular calcium stores in this process, we used thapsigargin, caffeine and ryanodine, all of which cause depletion of intracellular calcium stores. The PDGF effect was abolished using both thapsigargin and caffeine but not ryanodine. Collectively, these data indicate that in these human glioma cells PDGF-BB induces release of intracellular calcium from caffeine- and thapsigargin-sensitive calcium stores which in turn lead to further calcium influx through both NS and T channels.
- Subjects :
- medicine.medical_specialty
P-type calcium channel
Becaplermin
chemistry.chemical_element
Calcium-Transporting ATPases
Calcium
Endoplasmic Reticulum
omega-Conotoxins
Calcium in biology
Nicardipine
Cellular and Molecular Neuroscience
omega-Agatoxin IVA
Nitrendipine
omega-Conotoxin GVIA
Caffeine
Internal medicine
Tumor Cells, Cultured
medicine
Humans
Masoprocol
Calcium Signaling
Enzyme Inhibitors
Elapid Venoms
Platelet-Derived Growth Factor
Ion Transport
Voltage-dependent calcium channel
Brain Neoplasms
Ryanodine
Ryanodine receptor
Calcium channel
Imidazoles
T-type calcium channel
Glioma
Proto-Oncogene Proteins c-sis
Cell Biology
Calcium Channel Blockers
Flufenamic Acid
Neoplasm Proteins
Endocrinology
Verapamil
chemistry
Thapsigargin
Nimodipine
Calcium Channels
Peptides
medicine.drug
Subjects
Details
- ISSN :
- 01970186
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Neurochemistry International
- Accession number :
- edsair.doi.dedup.....71b971a20e6cdd49da22f1179401a0d0