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Neurologic Mechanisms Underlying Voiding Dysfunction due to Prostatitis in a Rat Model of Nonbacterial Prostatic Inflammation
- Source :
- International Neurourology Journal, Vol 22, Iss 2, Pp 90-98 (2018), International Neurourology Journal
- Publication Year :
- 2018
- Publisher :
- Korean Continence Society, 2018.
-
Abstract
- Purpose The neurological molecular mechanisms underlying the voiding dysfunction associated with nonbacterial chronic prostatitis/chronic pelvic pain syndrome remain poorly understood. In this study, we assessed whether prostate inflammation activated bladder afferent neurons, leading to bladder dysfunction, and sought to elucidate the underlying mechanisms. Methods Thirty male Sprague-Dawley rats were divided into 3 groups: sham-saline, formalin-injected, and capsaicin-pretreated and formalin-injected. Chemical prostatitis was induced by 0.1 mL of 10% buffered formalin injected into the ventral prostate. Capsaicin was injected subcutaneously to desensitize capsaicin-sensitive nerves. In each group, conscious cystometry was performed, and c-fos expression within the spinal cord was determined immunocytochemically. Double immunofluorescent staining with c-fos and choline acetyltransferase (ChAT) was performed. On the third day after pseudorabies virus (PRV) infection, c-fos and PRV double-staining was performed. Results Intraprostatic formalin significantly increased the maximal voiding pressure and decreased the intercontraction interval, compared with controls. Pretreatment with capsaicin significantly reversed these effects. More c-fos-positive cells were observed in the sacral parasympathetic nucleus (SPN) and dorsal gray commissure (DCM) in the prostatitis group than in the sham group. c-fos-positive cells decreased in the capsaicin-pretreated group. Preganglionic neurons labeled by c-fos and ChAT were observed in the SPN in rats with prostatitis. Interneurons labeled by c-fos and PRV were identified in the DCM after PRV infection. Conclusions Our results suggest that prostate inflammation activates afferent nerve fibers projecting to the lumbosacral spinal cord, producing reflex activation of spinal neurons innervating the bladder and bladder hyperreflexia. This is mediated by capsaicin-sensitive prostate afferent neurons.
- Subjects :
- Pathology
medicine.medical_specialty
Urology
Prostate afferent
030232 urology & nephrology
Prostatitis
Hyperreflexia
lcsh:RC870-923
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Prostate
medicine
medicine.diagnostic_test
Fundamental Science for Neurourology
business.industry
Cystometry
medicine.disease
Spinal cord
lcsh:Diseases of the genitourinary system. Urology
Choline acetyltransferase
Voiding dysfunction
medicine.anatomical_structure
Neurology
chemistry
Capsaicin
Reflex
Original Article
Neurology (clinical)
medicine.symptom
business
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20936931 and 20934777
- Volume :
- 22
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- International Neurourology Journal
- Accession number :
- edsair.doi.dedup.....71da3f5a72cd74fa0c1d16936e6030ad