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Comparative proteomic map among vanA-containing Enterococcus isolated from yellow-legged gulls
- Source :
- RIUR. Repositorio Institucional de la Universidad de La Rioja, instname
- Publication Year :
- 2012
- Publisher :
- Proteomass Scientific Society, 2012.
-
Abstract
- The increase of VRE, therefore, represents an urgent threat for patient care and creates a reservoir of mobile resistance genes for other, more virulent pathogens. The existence of VRE in different ecologic niches complicates the understanding of its epidemiology. The aim of the present study was to study the proteome of 2 vanA strains recovered from seagull faecal samples. The vanA E. durans and vanA E. faecium isolates presented different genomic patterns: tet(M)-tet(L)-erm(B) and tet(M)-tet(L)-erm(B)-hyl, respectively. A total of 123 spots were excised from two-dimensional gel electrophoresis (2-DE) gel of vanA E. durans SG 2 strain, and 16 were successfully identified by using MS, representing 42 different proteins. For the vanA E. faecium SG 50 strain, 93 spots were excised from the 2-DE gel and 23 were identified, representing 47 different proteins. The vancomycin/teicoplanin A-type resistance protein vanA in vanA E. durans SG 2 strain was present in two different spots. The identified proteins have shown diverse functional activities including glycolysis, conjugation, translation, protein biosynthesis, among others. This work reports the impact of proteomics on knowledge of vanA enterococci strains and will be helpful to further understand the antibiotic-resistant mechanism.
- Subjects :
- Proteomics
Virulence factors
biology
Bioinformatics
Computational biology
Antimicrobial resistance
biology.organism_classification
Biochemistry
Genome
Microbiology
Complement (complexity)
Databases
Antibiotic resistance
VanA-containing Enterococcus
Enterococcus
Proteome
Genetics
Molecular Biology
Organism
Subjects
Details
- ISSN :
- 21820287
- Volume :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of Integrated OMICS
- Accession number :
- edsair.doi.dedup.....71db986de5dfcf885b0a5fb4a5312107