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Direct cancer tissue proteomics: a method to identify candidate cancer biomarkers from formalin-fixed paraffin-embedded archival tissues
- Source :
- Oncogene. 26:65-76
- Publication Year :
- 2006
- Publisher :
- Springer Science and Business Media LLC, 2006.
-
Abstract
- Successful treatment of multiple cancer types requires early detection and identification of reliable biomarkers present in specific cancer tissues. To test the feasibility of identifying proteins from archival cancer tissues, we have developed a methodology, termed direct tissue proteomics (DTP), which can be used to identify proteins directly from formalin-fixed paraffin-embedded prostate cancer tissue samples. Using minute prostate biopsy sections, we demonstrate the identification of 428 prostate-expressed proteins using the shotgun method. Because the DTP method is not quantitative, we employed the absolute quantification method and demonstrate picogram level quantification of prostate-specific antigen. In depth bioinformatics analysis of these expressed proteins affords the categorization of metabolic pathways that may be important for distinct stages of prostate carcinogenesis. Furthermore, we validate Wnt-3 as an upregulated protein in cancerous prostate cells by immunohistochemistry. We propose that this general strategy provides a roadmap for successful identification of critical molecular targets of multiple cancer types.
- Subjects :
- Male
Proteomics
Cancer Research
Prostate biopsy
Molecular Sequence Data
Computational biology
Biology
medicine.disease_cause
Mass Spectrometry
Prostate cancer
Formaldehyde
Biomarkers, Tumor
Genetics
medicine
Humans
Amino Acid Sequence
Molecular Biology
Paraffin Embedding
medicine.diagnostic_test
Wnt signaling pathway
Computational Biology
Prostatic Neoplasms
Cancer
medicine.disease
Immunohistochemistry
Neoplasm Proteins
Prostate-specific antigen
Immunology
Cancer biomarkers
Carcinogenesis
Subjects
Details
- ISSN :
- 14765594 and 09509232
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Oncogene
- Accession number :
- edsair.doi.dedup.....71e73aca7f9e1d0079342d56afc06600