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Comparison of Gingival Crevicular Fluid Sampling Methods in Patients with Severe Chronic Periodontitis
- Source :
- Journal of Periodontology, Journal of Periodontology; Vol 82
- Publication Year :
- 2011
-
Abstract
- The analysis of samplings from periodontal pockets is important in the diagnosis and therapy of periodontitis. In this study, three different sampling techniques were compared to determine whether one method yielded samples suitable for the reproducible and simultaneous determination of bacterial load, cytokines, neutrophil elastase, and arginine-specific gingipains (Rgps). Rgps are an important virulence factor of Porphyromonas gingivalis, the exact concentration of which in gingival crevicular fluid (GCF) has not been quantified.GCF was sampled from four sites per patient (one sample per quadrant using two samples per method) in 36 patients with chronic periodontitis. One week later, the procedure was repeated with alternative methods. Variables determined were loads of Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans) and P. gingivalis, levels of interleukin-6 and -8, activity of neutrophil elastase, and level of Rgps.The detected cytokine levels were higher using paper strips compared to paper points. Bacteria were found in similar loads from paper strips and paper points. Rgps were only detectable in high quantities by washing the periodontal pocket. The level of Rgps correlated with the load of P. gingivalis.The use of paper strips was suitable for the simultaneous determination of microbial and immunologic parameters. Obtaining GCF by washing can be useful for special purposes. The gingipain concentration in periodontal pockets was directly determined to be ≤1.5 μM. This value indicated that most of the substrates of these proteases by in vitro assays identified until now can be easily degraded in P. gingivalis-infected sites.
- Subjects :
- Male
Gingival and periodontal pocket
Dentistry
Gastroenterology
Aggregatibacter actinomycetemcomitans
0302 clinical medicine
0303 health sciences
biology
Gingival Crevicular Fluid
Middle Aged
Cysteine Endopeptidases
Hemagglutinins
Neutrophil elastase
Gingipain Cysteine Endopeptidases
Periodontics
Female
Porphyromonas gingivalis
Adult
Paper
medicine.medical_specialty
Virulence Factors
Article
Specimen Handling
03 medical and health sciences
Internal medicine
Periodontal Attachment Loss
medicine
Humans
Periodontal Pocket
Adhesins, Bacterial
Therapeutic Irrigation
gingipain
030304 developmental biology
Periodontitis
business.industry
Interleukin-6
Interleukin-8
030206 dentistry
medicine.disease
biology.organism_classification
Chronic periodontitis
cytokines
Bacterial Load
Gingipain
gingival crevicular fluid
Actinobacillus
Chronic Periodontitis
biology.protein
business
Gingival Hemorrhage
Leukocyte Elastase
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of Periodontology, Journal of Periodontology; Vol 82
- Accession number :
- edsair.doi.dedup.....72085cb213d7e1df3470044baf4c0679