Additional file 9 of Mapping DNA interaction landscapes in psoriasis susceptibility loci highlights KLF4 as a target gene in 9q31

Additional file 9 : Figure S6. 3C-qPCR results in the 9q31.2 locus from the HindIII fragment containing the KLF4 gene and promoter. qPCR was carried out on HaCaT and My-La 3C libraries using TaqMan® as the reporter. The anchor fragment (distance 0) contained the entire KLF4 gene and promoter. An intergenic fragment located approximately 200 kb from the anchor fragment was utilised as a negative control region. Eleven test fragments were selected at regular intervals across the psoriasis association. The positive controls in the Dryden BrCa region were included. Asterisks denote fragments that had a significantly higher relative interaction frequency than the NCR (one-way ANOVA, adjusted P-value < 0.05). Bars show mean + SD of triplicate 3C libraries. Abbreviations: Int, intergenic; NCR, negative control region; BrCa, breast cancer.