Back to Search
Start Over
Phenotypic screening system using three-dimensional (3D) culture models for natural product screening
- Source :
- The Journal of Antibiotics. 74:660-666
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Recent progress in three-dimensional (3D) cell culture systems has attracted much attention in the fields of basic life science and drug development. Newly established methods include 3D co-culture, spheroid culture, and organoid culture; these methods enable more human tissue-like culture and have largely replaced traditional two-dimensional (2D) monolayer culture. By combining 3D culture methods with high-content imaging analysis, it is possible to obtain diverse and convincing data even during initial screening (which requires rapid and easy operating procedures). Until recently, 3D culture methods were considered expensive, time-consuming, complex, and unstable. However, by exploiting the self-assembling nature of cells and adding several technical improvements, we have developed several phenotypic screenings aimed at discovering anticancer compounds.
- Subjects :
- Pharmacology
Biological Products
Natural product
Phenotypic screening
Operating procedures
Cell Culture Techniques
Monolayer culture
Computational biology
Biology
Coculture Techniques
Cell Line
Imaging analysis
Organoids
chemistry.chemical_compound
chemistry
Drug development
Cell culture
Spheroids, Cellular
Drug Discovery
Animals
Humans
Subjects
Details
- ISSN :
- 18811469 and 00218820
- Volume :
- 74
- Database :
- OpenAIRE
- Journal :
- The Journal of Antibiotics
- Accession number :
- edsair.doi.dedup.....7241099adb17623bed8bcdf038bb7a2e