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Correlative Imaging of Trace Elements and Intact Molecular Species in a Single-Tissue Sample at the 50 μm Scale
- Source :
- Analytical chemistry. 93(40)
- Publication Year :
- 2021
-
Abstract
- Elemental and molecular imaging play a crucial role in understanding disease pathogenesis. To accurately correlate elemental and molecular markers, it is desirable to perform sequential elemental and molecular imaging on a single-tissue section. However, very little is known about the impact of performing these measurements in sequence. In this work, we highlight some of the challenges and successes associated with performing elemental mapping in sequence with mass spectrometry imaging. Specifically, the feasibility of molecular mapping using the mass spectrometry imaging (MSI) techniques matrix-assisted laser desorption ionization (MALDI) and desorption electrospray ionization (DESI) in sequence with the elemental mapping technique particle-induced X-ray emission (PIXE) is explored. Challenges for integration include substrate compatibility, as well as delocalization and spectral changes. We demonstrate that while sequential imaging comes with some compromises, sequential DESI-PIXE imaging is sufficient to correlate sulfur, iron, and lipid markers in a single tissue section at the 50 μm scale.
- Subjects :
- 0303 health sciences
Desorption electrospray ionization
Spectrometry, Mass, Electrospray Ionization
Chemistry
Electrospray ionization
010401 analytical chemistry
Analytical chemistry
Tissue sample
Mass spectrometry
01 natural sciences
Lipids
Mass spectrometry imaging
0104 chemical sciences
Analytical Chemistry
Molecular Imaging
Trace Elements
03 medical and health sciences
Matrix-assisted laser desorption/ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Correlative imaging
Molecular imaging
Sulfur
030304 developmental biology
Subjects
Details
- ISSN :
- 15206882 and 00032700
- Volume :
- 93
- Issue :
- 40
- Database :
- OpenAIRE
- Journal :
- Analytical chemistry
- Accession number :
- edsair.doi.dedup.....724af2ce06c1cb52f176d6454cd95c9d