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Protective role of tissue transglutaminase in the cell death induced by TNF-alpha in SH-SY5Y neuroblastoma cells
- Source :
- Journal of biochemistry and molecular biology. 37(2)
- Publication Year :
- 2004
-
Abstract
- Tissue transglutaminase (tTGase) regulates various biological processes, including extracellular matrix organization, cellular differentiation, and apoptosis. Here we report the protective role of tTGase in the cell death that is induced by the tumor necrosis factor alpha (TNF-alpha) and ceramide, a product of the TNF-alpha signaling pathway, in human neuroblastoma SH-SY5Y cells. Treatment with retinoic acid (RA) induced the differentiation of the neuroblastoma cells with the formation of extended neurites. Immunostaining and Western blot analysis showed the tTGase expression by RA treatment. TNF-alpha or C(2) ceramide, a cell permeable ceramide analog, induced cell death in normal cells, but cell death was largely inhibited by the RA treatment. The inhibition of tTGase by the tTGase inhibitors, monodansylcadaverine and cystamine, eliminated the protective role of RA-treatment in the cell death that is caused by TNF-alpha or C(2)-ceramide. In addition, the co-treatment of TNF-alpha and cycloheximide decreased the protein level of tTGase and cell viability in the RA-treated cells, supporting the role of tTGase in the protection of cell death. DNA fragmentation was also induced by the co-treatment of TNF-alpha and cycloheximide. These results suggest that tTGase expressed by RA treatment plays an important role in the protection of cell death caused by TNF-alpha and ceramide.
- Subjects :
- Ceramide
Programmed cell death
Tissue transglutaminase
Cell Survival
Cell
Retinoic acid
Cystamine
Apoptosis
Tretinoin
Ceramides
Biochemistry
chemistry.chemical_compound
Neuroblastoma
Cadaverine
Cell Line, Tumor
medicine
Humans
Cycloheximide
Enzyme Inhibitors
Molecular Biology
Neurons
Protein Synthesis Inhibitors
Transglutaminases
biology
Sh sy5y neuroblastoma
Chemistry
Tumor Necrosis Factor-alpha
Cell Differentiation
General Medicine
Lipid signaling
Immunohistochemistry
Cell biology
medicine.anatomical_structure
Cytoprotection
biology.protein
Cancer research
Tumor necrosis factor alpha
Subjects
Details
- ISSN :
- 12258687
- Volume :
- 37
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of biochemistry and molecular biology
- Accession number :
- edsair.doi.dedup.....725cc47d35a420cf7ef34fa625814927