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Vitamin D/VDR signaling induces miR-27a/b expression in oral lichen planus
- Source :
- Scientific Reports, Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
- Publication Year :
- 2020
- Publisher :
- Nature Publishing Group UK, 2020.
-
Abstract
- MicroRNA-27a/b are small non-coding RNAs which are reported to regulate inflammatory response and cell proliferation. Although some studies have demonstrated that miR-27b is down-regulated in the oral specimens of patients suffering with oral lichen planus (OLP), the molecular mechanism of miR-27b decrease remains a large mystery, and the expression of miR-27a in OLP is not well explored. Here, we demonstrated both miR-27a and miR-27b, compared with healthy controls, were reduced in the oral biopsies, serum and saliva samples derived from OLP patients. The reductions of miR-27a/b were also confirmed in the lipopolysaccharide (LPS)- or activated CD4+ T cell-treated human oral keratinocytes (HOKs). Furthermore, we found vitamin D receptor (VDR) binding sites in the promoters of miR-27a/b genes and verified this finding. We also tested miR-27a/b levels in the oral epithelium from paricalcitol-treated, vitamin D deficient or VDR knockout mice. In the rescue experiments, we confirmed vitamin D and VDR inhibited LPS- or activated CD4+ T cell-induced miR-27a/b reductions in HOKs. In sum, our results show that vitamin D/VDR signaling induces miR-27a/b in oral lichen planus.
- Subjects :
- Mucositis
Keratinocytes
Lipopolysaccharides
Saliva
Cell biology
Lipopolysaccharide
lcsh:Medicine
Down-Regulation
Calcitriol receptor
Article
chemistry.chemical_compound
Mice
stomatognathic system
Vitamin D and neurology
medicine
Animals
Humans
Vitamin D
lcsh:Science
Promoter Regions, Genetic
Mice, Knockout
Multidisciplinary
Binding Sites
Cell growth
lcsh:R
Promoter
Epithelial Cells
medicine.disease
Molecular biology
Mice, Inbred C57BL
stomatognathic diseases
Disease Models, Animal
MicroRNAs
chemistry
Case-Control Studies
Knockout mouse
Ergocalciferols
Receptors, Calcitriol
lcsh:Q
Oral lichen planus
Lichen Planus, Oral
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....726dd0aa98d90716e1f5a7ecdba44eb5