Isolation of Ribonucleotide-Peptide Complexes from Pasteurella multocida

SummaryRNA was extracted with 10% NaCl from acetone-dried cells of Past, mul-tocida and deproteinized by treatment with the chloroform-octanol mixture. After alkaline hydrolysis, CMP, AMP, UMP and GMP were separated by anion exchange chroma-tography and paper ionophoresis. The nu-cleotides were tightly bound to peptide material. Nucleotide-peptide complexes were also detected in the TCA-soluble extract from Past, multocida.