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Induction of γ-Interferon by Avarol in Human Peripheral Blood Lymphocytes

Authors :
Hans P. Laubenstein
Georg Hess
Karl-H. Meyer zum Büschenfelde
Werner E. G. Müller
Petra Reuter
Michael Bachmann
S. Rossol
Heinz C. Schröder
R. Voth
Source :
Japanese Journal of Cancer Research : Gann
Publication Year :
1988
Publisher :
Wiley, 1988.

Abstract

Avarol is a cytostatic and anti-human immunodeficiency virus (HIV) agent. In this study, the avarol caused induction of gamma-interferon (IFN-gamma) in buffy coat cells (human peripheral blood lymphocytes) is demonstrated by immunological and molecular biological techniques. IFN-gamma production was detected after a 24-hr incubation period with avarol; maximal production was obtained after 5 days in the presence of the optimal avarol concentration of 0.75 microgram/ml. Blotting experiments using human IFN-gamma cDNA and beta-actin cDNA containing plasmids showed that in the absence of avarol no IFN-gamma transcripts were present in lymphocytes. Already after a 24-hr incubation with avarol, IFN-gamma gene induction was detected, and maximal induction was found after a 5-day incubation period. The enhanced IFN-gamma production seems to be caused by a change at the transcriptional and/or post-transcriptional level, but not during subsequent nucleocytoplasmic transport of mRNA. This molecular event is specific, at least in relation to the expression of the beta-actin gene. Our studies demonstrate that avarol displays, besides its potential anti-tumor and anti-HIV activity, a potential immunomodulating effect.

Details

ISSN :
09105050
Volume :
79
Database :
OpenAIRE
Journal :
Japanese Journal of Cancer Research
Accession number :
edsair.doi.dedup.....72b4a40b941d62e03a81d0ebdf27c4ef
Full Text :
https://doi.org/10.1111/j.1349-7006.1988.tb00035.x