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Integration of Metabolite Profiling and Transcriptome Analysis Reveals Genes Related to Volatile Terpenoid Metabolism in Finger Citron (C. medica var. sarcodactylis)
- Source :
- Molecules, Vol 24, Iss 14, p 2564 (2019), Molecules, Volume 24, Issue 14
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Finger citron (Citrus medica var. sarcodactylis) is a popular ornamental tree and an important source of essential oils rich in terpenoids, but the mechanisms behind volatile formation are poorly understood. We investigated gene expression changes combined with volatile profiling of ten samples from three developing organs: flower, leaf, and fruit. A total of 62 volatiles were identified with limonene and &gamma<br />terpinene being the most abundant ones. Six volatiles were identified using partial least squares discriminant analysis (PLS-DA) that could be used as markers for distinguishing finger citron from other citrus species. RNA-Seq revealed 1,611,966,118 high quality clean reads that were assembled into 32,579 unigenes. From these a total of 58 terpene synthase (TPS) gene family members were identified and the spatial and temporal distribution of their transcripts was measured in developing organs. Transcript levels of transcription factor genes AP2/ERF (251), bHLH (169), bZIP (76), MYB (155), NAC (184), and WRKY (66) during finger citron development were also analyzed. From extracted subnetworks of three modules constructed by weighted gene co-expression network analysis (WGCNA), thirteen TPS genes and fifteen transcription factors were suggested to be related to volatile terpenoid formation. These results provide a framework for future investigations into the identification and regulatory network of terpenoids in finger citron.
- Subjects :
- 0106 biological sciences
Pharmaceutical Science
RNA-Seq
Flowers
Biology
01 natural sciences
Article
citrus
Analytical Chemistry
lcsh:QD241-441
03 medical and health sciences
food
lcsh:Organic chemistry
Gene Expression Regulation, Plant
Drug Discovery
terpene synthase gene
Oils, Volatile
Metabolomics
Gene family
Gene Regulatory Networks
MYB
Least-Squares Analysis
Physical and Theoretical Chemistry
γ-terpinene
Gene
transcription factor
Plant Proteins
030304 developmental biology
Genetics
0303 health sciences
Sequence Analysis, RNA
Terpenes
WGCNA
Gene Expression Profiling
Organic Chemistry
fungi
food and beverages
food.food
WRKY protein domain
Terpenoid
Citrus medica
Plant Leaves
Chemistry (miscellaneous)
Fruit
Molecular Medicine
limonene
Transcription Factor Gene
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 24
- Issue :
- 14
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....72dc1119e4b02742708b1c3c95a3813c