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Predicting Schwannoma Growth in a Tumor Model Using Targeted Imaging
- Source :
- Otol Neurotol
- Publication Year :
- 2021
-
Abstract
- INTRODUCTION: Vestibular schwannoma (VS) is a common pathology encountered in neurotology clinics. Many patients are observed with a “wait and scan” approach. Previous efforts to determine radiographic indicators of future growth have been unsuccessful. Using a mouse subcutaneous tumor model, we seek to determine if fluorescent imaging with directed immunotargets could be used to predict schwannoma growth rate. METHODS: Anti-VEGFR2 and anti-Her2/Neu monoclonal antibodies were covalently linked to a near-infrared probe (IRDye800). Immunodeficient mice underwent subcutaneous injections with a rat-derived schwann (R3) cell line. When tumor growth was evident, either Anti-VEGFR2-IRDye800, anti-Her2/Neu-IRDye800, or Immunoglobulin G (IgG) Isotype-IRDye800 (control) were injected via tail vein. The mice were serially imaged in a closed field near-IR device. Fluorescent data were analyzed for tumor signal and correlated with tumor sie and growth rate. Heterogeneity of fluorescent tumor signal was also assessed. RESULTS: In both anti-VEGFR2 and anti-Her2/Neu groups, there were strong correlations between day 1 mean tumor fluorescence and eventual maximum tumor volume (p = 0.002, 0.001; r(2) = 0.92, 0.86). There was also strong correlation with maximum tumor signal on day 1 and maximum tumor volume (p = 0.003, 0.008; r(2) = 0.90, 0.91). There was no such correlation in the control group (p = 0.99, 0.75; r(2) =0.0002, 0.028). CONCLUSION: Given the potential morbidity in VS intervention, observation is an appropriate approach for patients with slow-growing or stagnant tumors. We seek to identify immunotargets in a murine model that show promise in predicting schwannoma growth with advanced imaging techniques. Both Her2/Neu and VEGFR2 correlated strongly wth tumor size and growth rates and are promising targets that merit further investigation.
- Subjects :
- Diagnostic Imaging
Pathology
medicine.medical_specialty
medicine.drug_class
Radiography
Schwannoma
Monoclonal antibody
Immunoglobulin G
Article
Neurotology
Mice
Text mining
Cell Line, Tumor
medicine
Medical imaging
Animals
Humans
biology
business.industry
medicine.disease
Sensory Systems
Rats
Disease Models, Animal
Otorhinolaryngology
Cell culture
biology.protein
Neurology (clinical)
business
Neurilemmoma
Subjects
Details
- ISSN :
- 15374505
- Volume :
- 42
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Otologyneurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology
- Accession number :
- edsair.doi.dedup.....72e688b7fcf3c2b2f01b23a9ec032986