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The relationship between RUVBL1 (Pontin, TIP49, NMP238) and BCL6 in benign and malignant human lymphoid tissues

Authors :
Beverly W. Baron
Rebecca M. Baron
Joseph M. Baron
Source :
Biochemistry and Biophysics Reports
Publication Year :
2016
Publisher :
Elsevier BV, 2016.

Abstract

The human BCL6 gene, which is involved in the pathogenesis of certain human lymphomas, encodes a transcriptional repressor that is needed for germinal center B cell development and T follicular helper cell differentiation. Our goal was to identify BCL6 target genes using a cell system in which BCL6 repressive effects are inhibited followed by subtractive hybridization, and we detected the RUVBL1 (Pontin, TIP49) gene as a potential target of BCL6 repression. Here we show that the BCL6 protein significantly represses RUVBL1 transcription (6.8-fold). Knockdown of endogenous BCL6 in a human B cell lymphoma line leads to significant upregulation of RUVBL1, and there is an inverse expression pattern between the BCL6 and RUVBL1 proteins in certain human lymphomas. RUVBL1 is part of the AAA+ superfamily and participates in multiple processes, including gene transcription regulation, chromatin remodeling, and DNA repair, which, if dysregulated, may promote lymphoma development. A further understanding of the relationship between RUVBL1 and BCL6 should improve our understanding of the pathogenesis of human lymphomas.<br />Highlights • BCL6, a transcriptional repressor, is deregulated in human lymphomas. • The RUVBL1 (Pontin, TIP49) gene is a target of BCL6 repression. • Regulation of RUVBL1 by BCL6 may be important in lymphomagenesis.

Details

ISSN :
24055808
Volume :
6
Database :
OpenAIRE
Journal :
Biochemistry and Biophysics Reports
Accession number :
edsair.doi.dedup.....732a598bd9b23881906bfc48b3875244
Full Text :
https://doi.org/10.1016/j.bbrep.2016.02.006