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Antiviral effect of ovotransferrin in mouse peritoneal macrophages by up-regulating type I interferon expression
- Source :
- Food and Agricultural Immunology, Vol 29, Iss 1, Pp 600-614 (2018)
- Publication Year :
- 2018
- Publisher :
- Taylor & Francis Group, 2018.
-
Abstract
- Ovotransferrin (OVT) is one of the major proteins in avian albumen that exerts diverse biological activities. This study is aimed to investigate the antiviral effect and mechanism of OVT from the avian egg. Effects of OVT on vesicular stomatitis virus (VSV)-infected mouse peritoneal macrophage were detected by real-time quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, and western blot. In OVT-pretreated mouse peritoneal macrophages, results showed that replication of VSV was significantly suppressed and production of type I interferon (IFN) was increased. OVT down-regulated ring finger protein 125 (RNF125) production and up-regulated retinoic-acid-inducible gene I (RIG-I) expression by the nuclear factor-κB signalling pathway, resulting in the enhancement of the production of type I IFN. It demonstrated that the antiviral effect of OVT on innate immunity is enhanced by promoting type I IFN expression, indicating its potential as a functional food ingredient.
- Subjects :
- 0301 basic medicine
ovotransferrin
Agriculture (General)
Immunology
macrophage
antiviral effect
type i interferon
S1-972
03 medical and health sciences
Interferon
medicine
Macrophage
innate immunity
Innate immune system
030102 biochemistry & molecular biology
biology
Chemistry
Mechanism (biology)
Ovotransferrin
RC581-607
Cell biology
030104 developmental biology
biology.protein
Immunologic diseases. Allergy
human activities
Agronomy and Crop Science
Food Science
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 14653443 and 09540105
- Volume :
- 29
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Food and Agricultural Immunology
- Accession number :
- edsair.doi.dedup.....7337d1553265bf0a7fed9da3c931e0a5