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Telomeres are favoured targets of a persistent DNA damage response in ageing and stress-induced senescence
- Publication Year :
- 2012
- Publisher :
- Nature Publishing Group, 2012.
-
Abstract
- Telomeres are specialized nucleoprotein structures, which protect chromosome ends and have been implicated in the ageing process. Telomere shortening has been shown to contribute to a persistent DNA damage response (DDR) during replicative senescence, the irreversible loss of division potential of somatic cells. Similarly, persistent DDR foci can be found in stress-induced senescence, although their nature is not understood. Here we show, using immuno-fluorescent in situ hybridization and ChIP, that up to half of the DNA damage foci in stress-induced senescence are located at telomeres irrespective of telomerase activity. Moreover, live-cell imaging experiments reveal that all persistent foci are associated with telomeres. Finally, we report an age-dependent increase in frequencies of telomere-associated foci in gut and liver of mice, occurring irrespectively of telomere length. We conclude that telomeres are important targets for stress in vitro and in vivo and this has important consequences for the ageing process.
- Subjects :
- DNA Replication
Male
Senescence
Aging
Chromatin Immunoprecipitation
Telomerase
DNA Repair
Cell division
DNA repair
DNA damage
General Physics and Astronomy
Biology
General Biochemistry, Genetics and Molecular Biology
Cell Line
Mice
Animals
Humans
In Situ Hybridization, Fluorescence
Telomere Shortening
Multidisciplinary
DNA replication
General Chemistry
Telomere
Molecular biology
Cell biology
Gastrointestinal Tract
Mice, Inbred C57BL
Oxidative Stress
Liver
Ageing
Cell Division
DNA Damage
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....7344e0d742b58074e6be56b5a079fb6a