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A humin-dependent Dehalobacter species is involved in reductive debromination of tetrabromobisphenol A
A humin-dependent Dehalobacter species is involved in reductive debromination of tetrabromobisphenol A
- Source :
- Chemosphere. 92:1343-1348
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Tetrabromobisphenol A (TBBPA) is the most widely used brominated flame retardant on the market. It has been detected in various environmental samples, and a growing body of evidence has demonstrated its toxic effects on living organisms. In this study, we report the enrichment and phylogenetic identification of bacteria that debrominate TBBPA to bisphenol A in the presence of humin. Incubation experiments indicated that humin was required for this debromination activity. Of the five compounds examined for inclusion in the TBBPA-debrominating culture, formate was the optimal electron donor. A 16S rRNA gene library showed that the culture was dominated by three known dehalogenator genera: Dehalobacter, Geobacter, and Sulfurospirillum. Further investigation indicated that Dehalobacter was responsible for the debromination of TBBPA. PCR-denaturing gradient gel electrophoresis analysis showed that Dehalobacter grew in the culture by utilizing TBBPA. Moreover, the copy number of the Dehalobacter 16S rRNA genes increased by about two orders of magnitude in the cultures without the addition of TBBPA, whereas it increased by approximately four orders of magnitude when TBBPA was present. The incubation experiments showed that Dehalobacter was reliant on humin for its debromination activity, indicating a new type of metabolism in Dehalobacter that is linked to humin respiration.
- Subjects :
- Environmental Engineering
Halogenation
Health, Toxicology and Mutagenesis
Polybrominated Biphenyls
Dehalobacter
chemistry.chemical_compound
RNA, Ribosomal, 16S
Environmental Chemistry
Incubation
Humic Substances
Phylogeny
Flame Retardants
Gel electrophoresis
biology
Chemistry
Public Health, Environmental and Occupational Health
General Medicine
General Chemistry
biology.organism_classification
Pollution
Biodegradation, Environmental
Peptococcaceae
Environmental chemistry
Brominated flame retardant
Humin
Tetrabromobisphenol A
Environmental Pollutants
Bacteria
Geobacter
Subjects
Details
- ISSN :
- 00456535
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- Chemosphere
- Accession number :
- edsair.doi.dedup.....7351bfffdba513772ef2935e499c91af
- Full Text :
- https://doi.org/10.1016/j.chemosphere.2013.05.051