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Corrigendum: Double-flow focused liquid injector for efficient serial femtosecond crystallography
- Source :
- Scientific reports 7, 46846 (2017). doi:10.1038/srep46846
- Publication Year :
- 2017
- Publisher :
- Deutsches Elektronen-Synchrotron, DESY, Hamburg, 2017.
-
Abstract
- Scientific reports 7, 46846 (2017). doi:10.1038/srep46846<br />Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.<br />Published by Nature Publishing Group, London
- Subjects :
- ddc:000
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Scientific reports 7, 46846 (2017). doi:10.1038/srep46846
- Accession number :
- edsair.doi.dedup.....735d203b32f8a33bbb4893d0b3e99934
- Full Text :
- https://doi.org/10.3204/pubdb-2018-00518