Phosphate transport and the stoicheiometry of respiratory driven proton translocation in Escherichia coli

The stoicheiometry of respiratory driven proton translocation associated with the oxidation of endogenous substrates has been measured in an organic phosphate auxotrophic mutant of Escherichia coli . The results obtained indicate that movements of inorganic phosphate do not result in an experimental underestimation of the observed H+/site ratio in E. coli , as has previously been suggested for mitochondria.