Back to Search Start Over

Metagenomic approach reveals the fates and mechanisms of antibiotic resistance genes exposed to allicins during waste activated sludge fermentation: Insight of the microbial community, cellular status and gene regulation

Authors :
Jiashun Cao
Le Zhang
Jingyang Luo
Fang Fang
Yinglong Su
Wei Du
Yang Wu
Xiaoshi Cheng
Source :
Bioresource Technology. 342:125998
Publication Year :
2021
Publisher :
Elsevier BV, 2021.

Abstract

This work revealed the impacts of exogeneous allicins on the antibiotic resistance genes (ARGs) variations during waste activated sludge (WAS) fermentation process. The overall abundance of ARGs was respectively reduced by 4.84 and 9.42% in presence of 0.01 and 0.05 g allicin/g TSS. Allicins disrupted the EPS structure and increased the permeability of cell membranes, which resulted in the release of ARGs for subsequent removal. Allicins also reduced intracellular ATP levels, which was disadvantageous to ARGs dissemination. Besides, allicins affected the microbial community and decreased the abundance of potential hosts based on bacterial taxa-ARGs network analysis. Moreover, the metabolic pathways and genetic expressions (i.e., two-component system, quorum sensing, and SOS response) involved in ARGs propagation were down-regulated, which caused the ARGs alleviation in allicins-stressed reactors. Overall, the simultaneous responses of cellular status, bacterial host, and genetic regulation accounted for the effective ARGs reduction induced by allicins during WAS fermentation.

Details

ISSN :
09608524
Volume :
342
Database :
OpenAIRE
Journal :
Bioresource Technology
Accession number :
edsair.doi.dedup.....7363dd768e5b6ce3e0a900a60d29b657
Full Text :
https://doi.org/10.1016/j.biortech.2021.125998