Back to Search
Start Over
Establishment of a Seronegative Occult Infection With an Active Hepatitis B Virus Reservoir Enriched of Vaccine Escape Mutations in a Vaccinated Infant After Liver Transplantation
- Source :
- The Journal of Infectious Diseases. 220:1935-1939
- Publication Year :
- 2019
- Publisher :
- Oxford University Press (OUP), 2019.
-
Abstract
- We describe the establishment of a seronegative occult hepatitis B virus (HBV) infection (OBI) in a successfully vaccinated infant who underwent liver transplantation from an donor positive for antibody to hepatitis B core antigen (anti-HBc). The use of highly sensitive droplet digital polymerase chain reaction assays revealed a not negligible and transcriptionally active intrahepatic HBV reservoir (circular covalently closed DNA, relaxed circular DNA, and pregenomic RNA: 5.6, 2.4, and 1.1 copies/1000 cells, respectively), capable to sustain ongoing viral production and initial liver damage. Next-generation sequencing revealed a peculiar enrichment of hepatitis B surface antigen vaccine-escape mutations that could have played a crucial role in OBI transmission. This clinical case highlights the pathobiological complexity and the diagnostic challenges underlying OBI.
- Subjects :
- 0301 basic medicine
Hepatitis B virus
medicine.medical_treatment
Liver transplantation
Virus Replication
medicine.disease_cause
Polymerase Chain Reaction
Virus
hepatitis B occult infection
Settore MED/07
law.invention
HBV reservoir
digital droplet PCR
liver transplantation
vaccine escape mutations
03 medical and health sciences
0302 clinical medicine
Antigen
law
medicine
Humans
Immunology and Allergy
Polymerase chain reaction
biology
Transmission (medicine)
business.industry
Vaccination
Hepatitis B
Virology
030104 developmental biology
Infectious Diseases
Liver
Child, Preschool
DNA, Viral
Mutation
biology.protein
Female
030211 gastroenterology & hepatology
Antibody
business
Biomarkers
Subjects
Details
- ISSN :
- 15376613 and 00221899
- Volume :
- 220
- Database :
- OpenAIRE
- Journal :
- The Journal of Infectious Diseases
- Accession number :
- edsair.doi.dedup.....73b1fcbd49be73d261c645c2e8775db5