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Transcriptomic and GC-MS Metabolomic Analyses Reveal the Sink Strength Changes during Petunia Anther Development
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences; Volume 19; Issue 4; Pages: 955
- Publication Year :
- 2018
- Publisher :
- MDPI, 2018.
-
Abstract
- Petunia, which has been prevalently cultivated in landscaping, is a dicotyledonous herbaceous flower of high ornamental value. Annually, there is a massive worldwide market demand for petunia seeds. The normal development of anther is the necessary prerequisite for the plants to generate seeds. However, the knowledge of petunia anther development processes is still limited. To better understand the mechanisms of petunia anther development, the transcriptomes and metabolomes of petunia anthers at three typical development stages were constructed and then used to detect the gene expression patterns and primary metabolite profiles during the anther development processes. Results suggested that there were many differentially-expressed genes (DEGs) that mainly participated in photosynthesis and starch and sucrose metabolism when DEGs were compared between the different development stages of anthers. In this study, fructose and glucose, which were involved in starch and sucrose metabolism, were taken as the most important metabolites by partial least-squares discriminate analysis (PLS-DA). Additionally, the qRT-PCR analysis of the photosynthetic-related genes all showed decreased expression trends along with the anther development. These pieces of evidence indicated that the activities of energy and carbohydrate metabolic pathways were gradually reduced during all the development stages of anther, which affects the sink strength. Overall, this work provides a novel and comprehensive understanding of the metabolic processes in petunia anthers.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Stamen
Flowers
01 natural sciences
Petunia
Catalysis
Article
Gas Chromatography-Mass Spectrometry
Inorganic Chemistry
Transcriptome
anther development
03 medical and health sciences
Metabolomics
Gene Expression Regulation, Plant
Botany
Metabolome
Physical and Theoretical Chemistry
Molecular Biology
Spectroscopy
Petunia hybrida
transcriptome
metabolome
biology
Organic Chemistry
Primary metabolite
General Medicine
Herbaceous plant
biology.organism_classification
Computer Science Applications
Metabolic pathway
030104 developmental biology
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 19
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....73c663932cd93dc2ec463c23380f4749