Additional file 1 of Human breast microbiome correlates with prognostic features and immunological signatures in breast cancer

Additional file 1: Five supporting figures, with corresponding figure captions provided within the file. Figure S1. R code for removing ASVs detected in environmental/negative controls, and bacterial and non-eukaryotic ASVs characterized in environmental/negative controls. Figure S2. Total bacterial load is significantly higher in tissues versus environmental/negative controls and is similar across tissue types. Figure S3. Breast tumor tissue exhibits upregulation of multiple downstream toll-like receptor pathway genes. Figure S4. T-cell infiltration and association with specific bacterial genera vary by breast tissue type. Figure S5. Network analyses reveal microbiome–immune associations in high-risk and tumor adjacent normal breast tissues.